NOG mouse

(Formal name) NOD.Cg-Prkdcscid II2rgtm1Sug/Jic
(Abbreviated name) NOD/Shi-scid,IL-2Rnull
Japanese patent No. 3,753,321,US patent No. 7,145,055, EU patent No. 1,338,198
Japanese trademark registration No. 4,823,423, US trademark registration No. 3,118,040, EU trademark registration No. 3,736,758 For inquiries about the NOG mouse, contact

Development of the NOG mouse

The humanized mouse is a mouse harboring functioning human cells and/or tissues, used as an in vivo human model for both physiological and pathological conditions. This model is not only utilized as an applied research tool for drug discovery, pre-clinical tests and various human diseases, but is also used in basic research such as immunology, hematology and cancer. Humanized mice are being used by many researchers around the world and are making significant contributions to medical research.
The forerunner of the humanized mouse was the SCID-hu mouse (McCune JM et al., Science 1988) an immunodeficient SCID mouse (Bosma CG et al., Nature 1983) transplanted with human hematopoietic tissues. With the development of the NOD/scid mouse, an improved SCID mouse, it was possible to engraft more human cells and tissues than in the SCID mouse. However, many problems remained before an in vivo humanized model could be achieved.
Mamoru Ito and his group at the Laboratory Animal Research Department in CIEA were successful in establishing an extremely severe combined immunodeficient mouse called the NOG (NOD/Shi-scid,IL-2Rnull) mouse (Ito M et al., Blood 2002) by combining the NOD/scid mouse and the IL-2 receptor-chain (a common receptor for several cytokines) knockout (IL2rKO) mouse (Ohbo K. et al., Blood 1996).
The NOG mouse shows markedly better engraftment of human cells and human tissues than the NOD/scid mouse and also makes possible engraftment of human cancer cells, liver cells, etc. at high rates. In addition, after transplantation of human hematopoietic stem cells, human T cells can be developed in peripheral lymphoid tissues of the NOG mouse but not the NOD/scid mouse. Therefore, demand for the NOG mouse as a human immune system model mouse is increasing.

- Lacking T and B cells
- Lacking natural killer (NK) cells
- Reduced dendritic cell function
- Reduced macrophage function
- Lacking complement activity
- No T and B cell leakiness associated with aging

Humanized models using the NOG mouse

- Human hematopoietic system and human immune system model
- Human liver model
- Human cancer-bearing model
- Human infectious disease model
- GVHD model
- Other models

Care and management of NOG mice

- Precautions for handling NOG mice
- Basic data

References
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